FastQCFastQC Report
Thu 25 Nov 2021
HVTN5DSX2_l03_n02_HTO-CPAcore_K562-L2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTN5DSX2_l03_n02_HTO-CPAcore_K562-L2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52187302
Sequences flagged as poor quality0
Sequence length101
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCTATATACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3757140.719933749401339No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3684870.7060855531485418No Hit
TCCAATGCAACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3129970.5997570060241857No Hit
GACGTACCGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2654740.5086946246042764No Hit
TAACGACGTGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2595550.4973527851660161No Hit
GTGTGACGTATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2294400.4396471770086907No Hit
TAACGACGTGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2113900.4050602194380541No Hit
TATCACATCGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2100960.4025806890726024No Hit
CGCCTATATACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2041140.39111813061345846No Hit
CACATAATGACGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1753920.3360817541401163No Hit
ACTGTCTAACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1682380.3223734386575493No Hit
CGCCTATATACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1636500.3135820280573232No Hit
TCCAATGCAACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1553230.29762603937639853No Hit
TAACGACGTGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1529780.29313260915461775No Hit
GTGTGACGTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1364870.2615329683071181No Hit
TATCACATCGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1354760.2595957154481755No Hit
TCCAATGCAACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1324180.2537360524979812No Hit
CACATAATGACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1163690.22298336097160187No Hit
ACTGTCTAACGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1159480.22217665132410946No Hit
GACGTACCGGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1139360.2183213073555709No Hit
GTGTGACGTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1115260.21370332576303713No Hit
TATCACATCGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1107470.212210625488936No Hit
ACTGTCTAACGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1053340.20183837056761433No Hit
GACGTACCGGATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1018710.1952026567688822No Hit
CACATAATGACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA994920.19064407659932295No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)