FastQCFastQC Report
Thu 25 Nov 2021
HVTN5DSX2_l03_n02_HTO-CPAcore_K562-L1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTN5DSX2_l03_n02_HTO-CPAcore_K562-L1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52770314
Sequences flagged as poor quality0
Sequence length101
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCTATATACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3770330.7144793567080158No Hit
TCCAATGCAACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3534720.6698311478684776No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3471310.6578149222307073No Hit
GACGTACCGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2753850.5218559055760025No Hit
TAACGACGTGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2683210.5084695914449173No Hit
TAACGACGTGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2226430.42190956074280705No Hit
TATCACATCGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2211130.41901020335031547No Hit
GTGTGACGTATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2190300.41506290828589726No Hit
CGCCTATATACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2069950.39225652513646214No Hit
TCCAATGCAACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1762650.3340230266585111No Hit
ACTGTCTAACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1748840.3314060249859419No Hit
CACATAATGACGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1738690.3294825950817727No Hit
CGCCTATATACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1654720.31357023951003965No Hit
TAACGACGTGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1627170.3083495011987232No Hit
TCCAATGCAACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1488660.2821017892749321No Hit
TATCACATCGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1418730.2688500204868972No Hit
GTGTGACGTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1320550.2502448630493273No Hit
ACTGTCTAACGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1201170.2276222953685665No Hit
GACGTACCGGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1160490.21991341571323603No Hit
TATCACATCGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1150440.21800893585738373No Hit
CACATAATGACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1116850.2116436146277242No Hit
ACTGTCTAACGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1090280.20660858678991376No Hit
GTGTGACGTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1084460.20550569397786794No Hit
GACGTACCGGATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1040870.1972453679165146No Hit
CACATAATGACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA951370.1802850746728549No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)