FastQCFastQC Report
Thu 25 Nov 2021
HVTN5DSX2_l03_n02_GEX-CaRPool_PDL1-L2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTN5DSX2_l03_n02_GEX-CaRPool_PDL1-L2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122270818
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2754330.22526470707016943No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT1712610.1400669454914418Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)