FastQCFastQC Report
Thu 25 Nov 2021
HVTN5DSX2_l03_n02_ADT-CaRPool_PDL1-L2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTN5DSX2_l03_n02_ADT-CaRPool_PDL1-L2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216271233
Sequences flagged as poor quality0
Sequence length101
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA86066453.9795607028328175No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59253532.7397786186385686No Hit
CGTCACCTCTGTATCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43886932.0292541634513177No Hit
CTCCGAATCATGTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43513812.0120017533723495No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39855191.8428336236470246No Hit
AATAGCGAGCAAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39596011.830849598013805No Hit
CGCGCACCCATTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36724991.6980987018278109No Hit
TCAATCCTTCCGCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36401961.6831623649179455No Hit
TCAATCCTTCCGCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30891601.4283730467287805No Hit
TATATCCTTGTGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27316811.263081068206607No Hit
CTCCGAATCATGTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26715521.2352784801481203No Hit
ACAGTACGACCTTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24978761.1549737638939712No Hit
CGCGCACCCATTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24911171.1518485216200713No Hit
ATTCTGTCTTTCCCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24729771.1434609058709162No Hit
CGCGCACCCATTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23321711.0783546973165867No Hit
CTCCGAATCATGTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23257991.0754083970104336No Hit
CGTCACCTCTGTATCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22970461.0621135174274428No Hit
CGTCACCTCTGTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22763981.0525662467555268No Hit
TACGCCTATAACTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21862781.0108963497701982No Hit
ACAGTACGACCTTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21675601.002241477025287No Hit
AATAGCGAGCAAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20867920.9648957797359947No Hit
AATAGCGAGCAAGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16875730.7803039621085436No Hit
ATTCTGTCTTTCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16390600.757872407376528No Hit
TAGCGAGCCCGTATAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15011190.6940909242423379No Hit
ATTCTGTCTTTCCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14238470.6583617156332576No Hit
TACGCCTATAACTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13520080.6251446303078135No Hit
ACAGTACGACCTTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13450790.6219407830351621No Hit
TACGCCTATAACTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10875650.5028708556907335No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10098490.46693634932020756No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9852180.4555474097657731No Hit
TAGCGAGCCCGTATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9613770.44452375226436147No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT9233720.426950911219894No Hit
GCTCATTACCCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9150030.42308123336958087No Hit
TAGCGAGCCCGTATACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9143070.42275941525704436No Hit
GTTGTCCGACAATACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8614170.3983040129983445No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8440710.3902835288315945No Hit
GTTGTCCGACAATACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8049170.37217941047203446No Hit
ACAGCGCCGTATTTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7788510.3601269522516663No Hit
GTTGTCCGACAATACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7600350.35142676603688666No Hit
AAGTTCACTCTTTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7558720.3495018683321605No Hit
AAGTTCACTCTTTGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6627690.30645268480991184No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT5982370.2766142272837553No Hit
GCTCATTACCCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5766050.26661197238377055No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT5449120.2519576887047202No Hit
TCTCAGACCTCCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5248250.2426698145286849No Hit
CTCCGAATCATGTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT5033450.23273784174523107No Hit
GCTCATTACCCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4953220.22902814818649506No Hit
AATAGCGAGCAAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT4871620.22525510824641204No Hit
GACAAGTGATCTGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4809870.22239989726234186No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT4796960.22180296165417432No Hit
ACAGCGCCGTATTTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4623850.21379866086952024No Hit
CGTCACCTCTGTATCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT4504310.20827134231023692No Hit
TCAATCCTTCCGCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT4354130.20132728424404No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG4339300.20064157122551754No Hit
CGCGCACCCATTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT4320880.1997898629449253No Hit
TCAATCCTTCCGCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT4088690.1890538072624758No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC3920270.18126636379790742No Hit
TCTCAGACCTCCGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3810160.17617507179052333No Hit
ACAGCGCCGTATTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3638250.1682262568873411No Hit
GACAAGTGATCTGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3599840.16645024629789762No Hit
GACAAGTGATCTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3539240.16364820928357124No Hit
TATATCCTTGTGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT3376750.1561349585499427No Hit
CGCGCACCCATTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT3306810.15290105642482743No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG3243390.1499686275890423No Hit
TCTCAGACCTCCGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3239040.149767491268707No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT3219590.14886815760651811No Hit
ATTCTGTCTTTCCCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT3198590.14789715468076145No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA3067370.1418297735418191No Hit
CGCGCACCCATTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT3064580.14170076886739716No Hit
AAGTTCACTCTTTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2996660.13856026797609278No Hit
CTCCGAATCATGTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2993270.13840352036093492No Hit
CGTCACCTCTGTATCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2916240.13484178915279038No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT2830150.13086113953953368No Hit
ACAGTACGACCTTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2763990.1278020179410546No Hit
CTCCGAATCATGTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2746090.12697435354243344No Hit
CGTCACCTCTGTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2719900.12576337417931122No Hit
AATAGCGAGCAAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2701060.12489224584020381No Hit
AATAGCGAGCAAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2628230.12152471521721062No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2614890.12090789716818233No Hit
CTCCGAATCATGTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2578930.11924517025341044No Hit
CTCCGAATCATGTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT2554280.11810539777151038No Hit
ACAGTACGACCTTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2553760.11808135388953925No Hit
CTCCGAATCATGTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2510670.1160889483623557No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2466680.11405492842406831No Hit
CGCGCACCCATTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2401080.11102170023694274No Hit
TACGCCTATAACTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2397090.11083720968104897No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2353930.10884156747744625No Hit
AATAGCGAGCAAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT2352850.10879163018412162No Hit
AATAGCGAGCAAGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2329010.10768931067221502No Hit
AATAGCGAGCAAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2278400.10534919362114148No Hit
CGTCACCTCTGTATCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT2265510.10475318277766513No Hit
TCAATCCTTCCGCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT2259650.10448222672314446No Hit
TCAATCCTTCCGCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2221080.10269881801617138No Hit
TCAATCCTTCCGCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT2191110.10131305812641296No Hit
CGCGCACCCATTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2179150.10076004884107725No Hit
CGTCACCTCTGTATCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2171430.10040308967027528No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG2169010.10029119314264047No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)