FastQCFastQC Report
Thu 25 Nov 2021
HVTN5DSX2_l03_n02_ADT-CaRPool_PDL1-L1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTN5DSX2_l03_n02_ADT-CaRPool_PDL1-L1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204428803
Sequences flagged as poor quality0
Sequence length101
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA87094394.2603776337720864No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53600242.6219514673771287No Hit
CGTCACCTCTGTATCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42303272.0693400039132452No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41059442.008495838035113No Hit
CTCCGAATCATGTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39107121.9129946184736013No Hit
AATAGCGAGCAAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35336431.7285445828296515No Hit
TCAATCCTTCCGCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32569601.593200151937494No Hit
CGCGCACCCATTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32529551.5912410346598762No Hit
TCAATCCTTCCGCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27569771.3486245380011348No Hit
TATATCCTTGTGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27368291.338768783966318No Hit
ACAGTACGACCTTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25930341.26842889159802No Hit
CTCCGAATCATGTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23131761.1315313527516961No Hit
ATTCTGTCTTTCCCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22834381.1169844789435077No Hit
ACAGTACGACCTTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22440231.0977039277581642No Hit
CGCGCACCCATTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22375091.0945174883208606No Hit
CGTCACCTCTGTATCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22116901.081887663354366No Hit
TACGCCTATAACTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21938491.0731604195716No Hit
CGTCACCTCTGTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21909801.0717569969824652No Hit
CGCGCACCCATTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20821731.0185321096851503No Hit
CTCCGAATCATGTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20740971.0145815900511828No Hit
AATAGCGAGCAAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18633500.9114909311482884No Hit
ATTCTGTCTTTCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15149490.7410643597027763No Hit
AATAGCGAGCAAGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15066280.7369939939432116No Hit
ACAGTACGACCTTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13961910.6829717630347814No Hit
TACGCCTATAACTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13226270.6469866186126424No Hit
TAGCGAGCCCGTATAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13209210.646152098244199No Hit
ATTCTGTCTTTCCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13125300.6420474907344637No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11216860.5486927397407889No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10929680.5346448171493721No Hit
TACGCCTATAACTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10761610.5264233729334119No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9374490.4585699207953588No Hit
ACAGCGCCGTATTTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9349090.45732743443202567No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT9040060.4422106800674267No Hit
TAGCGAGCCCGTATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8457170.41369757470037133No Hit
GTTGTCCGACAATACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8439020.4128097350352337No Hit
GCTCATTACCCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8309120.4064554445392903No Hit
TAGCGAGCCCGTATACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8023180.39246817876246143No Hit
GTTGTCCGACAATACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7832450.3831382801766931No Hit
GTTGTCCGACAATACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7397140.36184431408131856No Hit
TCTCAGACCTCCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7275400.3558891845587923No Hit
AAGTTCACTCTTTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7067560.3457223197652828No Hit
AAGTTCACTCTTTGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6243230.30539874559652924No Hit
ACAGCGCCGTATTTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5574120.27266803494417563No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT5494130.2687551812353957No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT5314310.25995896478442915No Hit
GCTCATTACCCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5247520.25669181265029467No Hit
TCTCAGACCTCCGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4908550.24011048971411333No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT4575870.22383685336160777No Hit
GCTCATTACCCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4503200.2202820705260403No Hit
CTCCGAATCATGTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT4427630.21658542901119465No Hit
GACAAGTGATCTGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4389750.21473246115910585No Hit
ACAGCGCCGTATTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4334860.2120474187778715No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG4329730.211796475665907No Hit
AATAGCGAGCAAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT4261980.2084823634172529No Hit
CGTCACCTCTGTATCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT4233820.20710486672467576No Hit
TCTCAGACCTCCGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4141780.20260256574510196No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC3869070.18926246904649732No Hit
TCAATCCTTCCGCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT3809580.18635240944985626No Hit
CGCGCACCCATTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT3772280.18452781333362303No Hit
TCAATCCTTCCGCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT3579310.1750883411473089No Hit
GACAAGTGATCTGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3274590.16018241813018883No Hit
TATATCCTTGTGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT3265660.15974559123158394No Hit
GACAAGTGATCTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3239200.1584512530751354No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT3164850.15481429003915853No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA3057830.14957921560593396No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2941990.14391269512055987No Hit
ATTCTGTCTTTCCCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2905080.1421071765508503No Hit
CGCGCACCCATTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2900020.14185965761390287No Hit
ACAGTACGACCTTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2815970.13774820175413344No Hit
AAGTTCACTCTTTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2753520.13469334847105668No Hit
CGTCACCTCTGTATCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2735430.13380844381307658No Hit
CGCGCACCCATTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2706200.1323786061595244No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2640980.129188253379344No Hit
CTCCGAATCATGTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2626990.12850390754379168No Hit
ACAGTACGACCTTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2603410.12735044973090215No Hit
CGTCACCTCTGTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2530210.12376974099877697No Hit
AATAGCGAGCAAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2455850.1201322887949405No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT2450330.11986226813645237No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2431190.11892600085321636No Hit
CTCCGAATCATGTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2428640.11880126304902347No Hit
TACGCCTATAACTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2316610.11332111551814936No Hit
AATAGCGAGCAAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2306660.11283439349786732No Hit
CTCCGAATCATGTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2304840.11274536494742377No Hit
CTCCGAATCATGTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2263370.11071678583374574No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2212790.10824257479999039No Hit
CTCCGAATCATGTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT2189980.10712678291228854No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA2186890.10697563004367834No Hit
CGCGCACCCATTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2174510.10637004023351837No Hit
CGTCACCTCTGTATCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2121690.1037862555992171No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG2120780.10374174132399533No Hit
CGTCACCTCTGTATCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT2064170.10097256207091326No Hit
CGTCACCTCTGTATCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2057920.10066683215867579No Hit
AATAGCGAGCAAGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2056110.10057829277609182No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)