FastQCFastQC Report
Thu 25 Nov 2021
HVTN5DSX2_l02_n02_HTO-CaRPool_THP1-L4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTN5DSX2_l02_n02_HTO-CaRPool_THP1-L4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54042422
Sequences flagged as poor quality0
Sequence length101
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6533341.208928052854478No Hit
TAACGACGTGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1323870.24496866554204402No Hit
TATCACATCGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1262940.23369418935368957No Hit
TAACGACGTGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1094300.20248907423135107No Hit
ACATGTTACCGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1053870.19500791433811016No Hit
CGCCTATATACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1019330.1886166389803921No Hit
ACATGTTACCGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA953610.17645582205771607No Hit
TAACGACGTGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA947980.1754140478752044No Hit
GACGTACCGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA840570.1555389208870024No Hit
TATCACATCGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA829570.15350348287499033No Hit
TATCACATCGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA801600.14832791912990131No Hit
ACTGTCTAACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA780920.14450129566731854No Hit
ACATGTTACCGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA773460.14312089861553576No Hit
TCCAATGCAACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA757810.14022502544390036No Hit
CACATAATGACGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA751160.13899451064572937No Hit
CGCCTATATACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA685380.12682259133389692No Hit
AGGACCATCCAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA624450.11554811514554252No Hit
ACTGTCTAACGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA577010.10676982611919207No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)