FastQCFastQC Report
Thu 25 Nov 2021
HVTN5DSX2_l02_n02_HTO-CaRPool_THP1-L3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTN5DSX2_l02_n02_HTO-CaRPool_THP1-L3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45643144
Sequences flagged as poor quality0
Sequence length101
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6296171.3794338970163844No Hit
TAACGACGTGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1095860.2400930137503236No Hit
TATCACATCGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1090570.23893402259931962No Hit
TAACGACGTGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA864160.18932963951825932No Hit
ACATGTTACCGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820580.17978165570715288No Hit
CGCCTATATACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA816120.17880451004865044No Hit
TAACGACGTGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770880.1688928352525409No Hit
ACATGTTACCGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA764580.16751256223716754No Hit
GACGTACCGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA740540.16224561568326668No Hit
TATCACATCGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA703730.15418087763629956No Hit
TATCACATCGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA673240.14750079442380218No Hit
ACTGTCTAACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA667780.1463045578104786No Hit
CACATAATGACGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA645450.14141225678932198No Hit
ACATGTTACCGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA616700.13511339183821341No Hit
TCCAATGCAACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA589320.12911468149520988No Hit
CGCCTATATACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA542620.1188831339050614No Hit
ACTGTCTAACGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA512240.11222715069759437No Hit
AGGACCATCCAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA506320.11093013224505305No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)