FastQCFastQC Report
Thu 25 Nov 2021
HVTN5DSX2_l02_n02_ADT-CaRPool_THP1-L4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTN5DSX2_l02_n02_ADT-CaRPool_THP1-L4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322679446
Sequences flagged as poor quality0
Sequence length101
%GC27

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA103144733.196507595342778No Hit
TATATCCTTGTGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55543731.72132841705697No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47995991.4874201190986303No Hit
TAACTCAGGGCCTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45479871.4094442817408332No Hit
TAACTCAGGGCCTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36018101.1162192214746767No Hit
CCTATTCCGATTCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30924430.958363799843638No Hit
AATAGCGAGCAAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27848060.8630255302967145No Hit
TAACTCAGGGCCTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26829240.8314517807868059No Hit
CCTATTCCGATTCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24439060.7573788880249905No Hit
TACGCCTATAACTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22859260.7084200832550084No Hit
GAGATGTCTGCAACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22533930.6983379412396785No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT22186020.6875560335503985No Hit
GAGATGTCTGCAACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21969230.6808376012892994No Hit
CCTATTCCGATTCCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19830730.6145643996178176No Hit
AATAGCGAGCAAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19417900.601770588139661No Hit
TACGCCTATAACTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16736920.5186856556088174No Hit
GCGATGGTAGATTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13990510.4335730141299425No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT13733870.4256196101192017No Hit
GACAAGTGATCTGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13687640.4241869189275849No Hit
TATATCCTTGTGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT13506440.4185714388514229No Hit
AATAGCGAGCAAGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13246140.41050460958086554No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT12346520.3826249286420307No Hit
GAGATGTCTGCAACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12121710.3756579525055959No Hit
GACAAGTGATCTGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12055780.37361474830349123No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10862080.3366213787289073No Hit
TACGCCTATAACTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10721350.33226008451743777No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10145740.31442163812317936No Hit
GACAAGTGATCTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10056310.31165015697962983No Hit
CGTCACCTCTGTATCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9520010.2950299474606139No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9218280.28567918143754345No Hit
TAACTCAGGGCCTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT8971090.278018637728788No Hit
TATATCCTTGTGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT8893590.2756168733474273No Hit
GCGATGGTAGATTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8842860.27404472486915077No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT8469110.26246202244936295No Hit
TAACTCAGGGCCTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT7602010.23559015283545515No Hit
CCTATTCCGATTCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT7330700.22718211807020394No Hit
TATATCCTTGTGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7271990.22536266533691768No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7227970.2239984631683048No Hit
GAGCTACTTCACTCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6926220.21464707733507143No Hit
AATAGCGAGCAAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT6887860.21345828144256826No Hit
GCGATGGTAGATTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6868230.21284993776765065No Hit
GAGCTACTTCACTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6785840.2102966298014532No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA6702020.20769900540860603No Hit
CGTCACCTCTGTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6431130.1993039866567764No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC6347090.19669954435213702No Hit
TAGCGAGCCCGTATAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6177350.1914392154993349No Hit
GAGATGTCTGCAACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT6132560.19005115063944916No Hit
TAACTCAGGGCCTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT6080340.18843282630403427No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT5928340.18372226906575265No Hit
CCTATTCCGATTCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT5745540.17805720417655607No Hit
AAGTCACCCGCTTTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5561010.1723385257082659No Hit
TAGCGAGCCCGTATACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5420500.1679840494085886No Hit
CTTCACTCTGTCAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5352950.16589064058328648No Hit
CCTATTCCGATTCCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT5246720.1625985189028743No Hit
TACGCCTATAACTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT5193910.16096191016765288No Hit
AAGTCACCCGCTTTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5130540.1589980416664035No Hit
GAGATGTCTGCAACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT5121910.15873059358109845No Hit
TAACTCAGGGCCTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT5000710.15497454399373178No Hit
AATAGCGAGCAAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT4913900.15228425798152634No Hit
TCAATCCTTCCGCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4781150.14817026802506658No Hit
TAACTCAGGGCCTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT4558580.14127271062688015No Hit
TATATCCTTGTGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA4554550.1411478188790494No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA4496920.13936183589456144No Hit
TATATCCTTGTGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC4406060.13654603832436232No Hit
CGTCACCTCTGTATCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4375660.135603926876706No Hit
TATATCCTTGTGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT4261770.1320744179038909No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC4223470.13088748144187654No Hit
CTTTGTACGAGCAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4161490.1289666897469509No Hit
CCTATTCCGATTCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT4140320.1283106206894876No Hit
CTTCACTCTGTCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4078790.12640377472322795No Hit
GAGATGTCTGCAACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT4061650.1258725974135954No Hit
CTTTGTACGAGCAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3975440.12320090570627792No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT3942390.12217666941203315No Hit
AATAGCGAGCAAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT3933660.12190612227591341No Hit
CTTTGTACGAGCAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3790550.117471070655055No Hit
TAACTCAGGGCCTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT3744010.11602877240591272No Hit
TAGCGAGCCCGTATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3725030.11544057256129045No Hit
AATAGCGAGCAAGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT3621150.1122212785750227No Hit
TACGCCTATAACTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT3611390.11191881121551199No Hit
CCTATTCCGATTCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT3486000.10803291139901115No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTA3446980.10682366177113121No Hit
GAGCTACTTCACTCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3421760.10604208115567422No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA3399110.10534014614615397No Hit
CCTATTCCGATTCCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT3363910.10424928025939402No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)