FastQCFastQC Report
Thu 25 Nov 2021
HVTN5DSX2_l02_n02_ADT-CaRPool_THP1-L3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTN5DSX2_l02_n02_ADT-CaRPool_THP1-L3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332821727
Sequences flagged as poor quality0
Sequence length101
%GC27

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA109065483.276993992642794No Hit
TATATCCTTGTGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58953661.771328468588831No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50409781.5146180645832656No Hit
TAACTCAGGGCCTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46331111.3920698752939287No Hit
TAACTCAGGGCCTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37117871.1152478035185485No Hit
CCTATTCCGATTCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31916810.9589761548229693No Hit
AATAGCGAGCAAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28822680.8660095679390547No Hit
TAACTCAGGGCCTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27326420.8210527673873887No Hit
CCTATTCCGATTCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25348260.7616167438491779No Hit
TACGCCTATAACTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24007520.7213327151565438No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT23010260.691368926163886No Hit
GAGATGTCTGCAACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22794900.684898194762387No Hit
GAGATGTCTGCAACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22287640.6696570022905987No Hit
CCTATTCCGATTCCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20374100.6121625587262216No Hit
AATAGCGAGCAAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20327900.610774428197111No Hit
TACGCCTATAACTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17510550.5261240051194134No Hit
GCGATGGTAGATTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14182790.4261377443065789No Hit
TATATCCTTGTGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT14085690.42322026650621886No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT14072900.4228359766909088No Hit
AATAGCGAGCAAGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13823850.4153529916633117No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT12870030.38669440592140186No Hit
GAGATGTCTGCAACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12312430.3699406920029593No Hit
TACGCCTATAACTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11206500.3367117916553567No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10951980.32906445437680215No Hit
GACAAGTGATCTGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10911100.3278361691813468No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10181280.30590791327754874No Hit
CGTCACCTCTGTATCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9745750.2928219286597236No Hit
GACAAGTGATCTGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9383450.2819362210688847No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9357080.28114390500713915No Hit
TATATCCTTGTGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT9199860.2764200547520144No Hit
TAACTCAGGGCCTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT9038280.27156520343396934No Hit
GCGATGGTAGATTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9017280.2709342350116464No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT8730370.262313704056947No Hit
GACAAGTGATCTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8063610.24228015618703883No Hit
TATATCCTTGTGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7926540.23816173515619066No Hit
TAACTCAGGGCCTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT7734130.23238056210194477No Hit
CCTATTCCGATTCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT7465840.22431948981503844No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7388850.22200623939434097No Hit
GAGCTACTTCACTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7149560.21481650445254735No Hit
GAGCTACTTCACTCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7126840.2141338567118246No Hit
AATAGCGAGCAAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT7114920.2137757070168679No Hit
GCGATGGTAGATTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7014280.2107518659681734No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA6999270.21030087377678924No Hit
CGTCACCTCTGTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6663090.20019997071885876No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC6537860.19643729569373938No Hit
GAGATGTCTGCAACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT6234790.1873312195150048No Hit
TAGCGAGCCCGTATAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6196350.18617624684100026No Hit
TAACTCAGGGCCTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT6139570.18447022841150032No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT6062510.18215487476272846No Hit
CCTATTCCGATTCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT5871760.17642357825996136No Hit
AAGTCACCCGCTTTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5674250.170489170017437No Hit
CTTCACTCTGTCAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5673930.1704795552605254No Hit
CTTTGTACGAGCAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5596670.1681581923886838No Hit
TAGCGAGCCCGTATACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5520410.16586687563219094No Hit
CCTATTCCGATTCCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT5349980.1607461161933097No Hit
AAGTCACCCGCTTTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5327340.16006587214181484No Hit
TACGCCTATAACTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT5309180.1595202346870822No Hit
GAGATGTCTGCAACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT5229610.15712946528878508No Hit
AATAGCGAGCAAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT5124740.1539785291721655No Hit
CTTTGTACGAGCAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5098950.1532036398573222No Hit
TAACTCAGGGCCTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT4976440.1495226902659513No Hit
TCAATCCTTCCGCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4938410.14838003649923973No Hit
CTTTGTACGAGCAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4933200.14822349623827294No Hit
TATATCCTTGTGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA4796520.1441167931924108No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA4727890.14205472829602858No Hit
TAACTCAGGGCCTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT4593120.13800541333048247No Hit
TATATCCTTGTGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC4579180.1375865704825214No Hit
CGTCACCTCTGTATCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4530090.13611160668005307No Hit
TATATCCTTGTGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT4421800.1328579128489409No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC4376860.13150764042516971No Hit
CTTCACTCTGTCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4223090.12688744926799805No Hit
CCTATTCCGATTCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT4208200.12644006261045573No Hit
GAGATGTCTGCAACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT4123690.12390086540233596No Hit
AATAGCGAGCAAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT4056060.12186884662130247No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT4048650.12164620490656848No Hit
AATAGCGAGCAAGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT3778310.11352353808319732No Hit
TACGCCTATAACTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT3757880.1129096959466231No Hit
TAACTCAGGGCCTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT3755330.11283307835248388No Hit
TAGCGAGCCCGTATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3735370.11223335789012356No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA3568620.1072231681557256No Hit
GAGCTACTTCACTCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3547100.10657657575342129No Hit
CCTATTCCGATTCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT3532240.10613009047933941No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTA3452930.10374713307103295No Hit
CCTATTCCGATTCCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT3421180.1027931689087113No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)