FastQCFastQC Report
Thu 25 Nov 2021
HVTN5DSX2_l01_n02_HTO-CaRPool_THP1-L2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTN5DSX2_l01_n02_HTO-CaRPool_THP1-L2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48177484
Sequences flagged as poor quality0
Sequence length101
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7420391.5402194934048445No Hit
TAACGACGTGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1280030.2656905038876667No Hit
TATCACATCGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1275380.2647253227254458No Hit
TAACGACGTGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1040740.21602207371393656No Hit
CGCCTATATACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1004730.20854762776736122No Hit
ACATGTTACCGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA995150.20655914700734473No Hit
ACATGTTACCGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA845970.17559447479656679No Hit
TAACGACGTGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA810490.16823003874590045No Hit
GACGTACCGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA808070.1677277294098629No Hit
TATCACATCGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA791540.16429666605254853No Hit
ACTGTCTAACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA754690.15664786479924936No Hit
TATCACATCGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA751910.1560708317603302No Hit
ACATGTTACCGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA748900.15544605857790333No Hit
CACATAATGACGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA731380.1518095050376645No Hit
TCCAATGCAACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA674660.14003637051698256No Hit
CGCCTATATACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA625370.12980545019744077No Hit
AGGACCATCCAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA598620.12425306394165375No Hit
ACTGTCTAACGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA529380.10988120508742218No Hit
ACTGTCTAACGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510620.10598726990392442No Hit
AGGACCATCCAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA485920.10086039362287993No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)