FastQCFastQC Report
Thu 25 Nov 2021
HVTN5DSX2_l01_n02_ADT-CaRPool_THP1-L2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTN5DSX2_l01_n02_ADT-CaRPool_THP1-L2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382897616
Sequences flagged as poor quality0
Sequence length101
%GC27

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA145654933.804017677665562No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67966061.775045264319431No Hit
TATATCCTTGTGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63897231.668781087422597No Hit
TAACTCAGGGCCTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63892601.66866016736965No Hit
TAACTCAGGGCCTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50433531.3171544531110375No Hit
CCTATTCCGATTCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44925331.1732987650672653No Hit
AATAGCGAGCAAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38212750.9979887156048525No Hit
TAACTCAGGGCCTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37916820.9902600177066654No Hit
CCTATTCCGATTCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33605220.8776555036059561No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT31398980.8200359231278159No Hit
GAGATGTCTGCAACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30675630.8011444500610315No Hit
GAGATGTCTGCAACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30668710.8009637228976636No Hit
TACGCCTATAACTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29390930.7675923999485021No Hit
CCTATTCCGATTCCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28097020.7337998155621841No Hit
AATAGCGAGCAAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24537450.6408357998238359No Hit
TACGCCTATAACTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20071180.5241918246887178No Hit
GCGATGGTAGATTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19798300.5170651153910554No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT19202380.5015016860277344No Hit
AATAGCGAGCAAGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18249630.47661905526202075No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT17766630.46400471712521707No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16246670.4243084657910223No Hit
TATATCCTTGTGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT15741730.41112112852643096No Hit
GACAAGTGATCTGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15576660.4068100544141283No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15157460.39586195804363533No Hit
TACGCCTATAACTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13710750.3580787507436453No Hit
GAGATGTCTGCAACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13562960.3542189722069202No Hit
GACAAGTGATCTGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13543190.35370264619250075No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13536310.35352296369481706No Hit
TAACTCAGGGCCTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT12791640.33407468381835004No Hit
CGTCACCTCTGTATCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12154070.31742349631134814No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT12109250.3162529484121938No Hit
GACAAGTGATCTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11326740.29581641479846665No Hit
GCGATGGTAGATTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10869840.28388372102060827No Hit
CCTATTCCGATTCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT10840920.2831284277309264No Hit
TAACTCAGGGCCTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT10818330.2825384527857703No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10402750.27168489865969814No Hit
TATATCCTTGTGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT10229770.26716724190834346No Hit
GCGATGGTAGATTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10112050.26409279079972126No Hit
AATAGCGAGCAAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT9638390.25172238210018005No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA9135820.2385969412773779No Hit
TAGCGAGCCCGTATAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9002600.23511768221612536No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC8907580.23263607888329083No Hit
TAACTCAGGGCCTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT8769870.22903955609898602No Hit
GAGATGTCTGCAACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT8678740.22665954650394066No Hit
GAGCTACTTCACTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8522640.2225827386713215No Hit
GAGCTACTTCACTCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8423350.2199896172766978No Hit
CCTATTCCGATTCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT8028940.20968895246399238No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT7986200.2085727271804168No Hit
CCTATTCCGATTCCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT7651560.19983305406633822No Hit
CGTCACCTCTGTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7343140.1917781593082575No Hit
TAACTCAGGGCCTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT7113890.18579091910564416No Hit
GAGATGTCTGCAACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT7080430.1849170562608047No Hit
TAGCGAGCCCGTATACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6796240.1774949677409326No Hit
TACGCCTATAACTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT6697350.174912293003151No Hit
AAGTCACCCGCTTTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6681480.17449782189294175No Hit
CTTTGTACGAGCAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6502420.1698213759575876No Hit
TAACTCAGGGCCTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT6475910.16912902377537914No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA6358000.16604961050475697No Hit
AATAGCGAGCAAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT6343950.16568267168317913No Hit
TCAATCCTTCCGCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6124570.15995320273814395No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC6081470.1588275754634106No Hit
CCTATTCCGATTCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT6044550.1578633490368872No Hit
TATATCCTTGTGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5915550.1544943022053185No Hit
TAGCGAGCCCGTATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5845630.15266822658932408No Hit
GAGATGTCTGCAACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT5764820.1505577407408042No Hit
CGTCACCTCTGTATCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5731990.14970033138049102No Hit
CTTTGTACGAGCAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5655340.1476984907631287No Hit
CTTTGTACGAGCAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5477040.14304189347577448No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT5468930.14283008750830142No Hit
AATAGCGAGCAAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT5421560.1415929421717789No Hit
TAACTCAGGGCCTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT5418780.1415203379067265No Hit
CTTCACTCTGTCAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5278610.1378595681828429No Hit
AAGTCACCCGCTTTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5172420.13508624195769345No Hit
AATAGCGAGCAAGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT5143680.1343356496635905No Hit
TATATCCTTGTGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA5085440.1328146164273846No Hit
TATATCCTTGTGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC4957700.12947847656486844No Hit
CCTATTCCGATTCCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT4855420.12680726641035028No Hit
CCTATTCCGATTCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT4830230.12614938819572072No Hit
TATATCCTTGTGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT4681180.1222567026899431No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA4571400.11938961771963605No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTA4530420.11831935772616564No Hit
CTTCACTCTGTCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4499830.11752044964416808No Hit
TCAATCCTTCCGCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4427290.11562594842585805No Hit
TACGCCTATAACTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT4388230.11460583238522959No Hit
TAACTCAGGGCCTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC4346150.11350684408544345No Hit
GCGATGGTAGATTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT4259840.11125271670534506No Hit
GAGATGTCTGCAACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT4219820.11020752868829563No Hit
GAGCTACTTCACTCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4108480.10729970175630447No Hit
ATCCTTTGCCGTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4106070.10723676064883099No Hit
AATAGCGAGCAAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT4039770.10550522727725734No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG3930050.10263970930547658No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAA3928720.10260497417147668No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT3889740.10158694746221664No Hit
TAACTCAGGGCCTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC3832420.10008994153674752No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)