FastQCFastQC Report
Thu 7 Sep 2017
HVTL7BGX2_n01_tga1ox_60n_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTL7BGX2_n01_tga1ox_60n_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6250800
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG94040.1504447430728867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG20200.035.011818
TGTGCGA24350.029.1849889
GTGCGAG24450.028.92475710
TGTGTGC26500.026.8182227
CTGACAT28450.026.5634921
TGCGAGT27200.026.12711
GCCAGTA16400.023.9874611
ACATGTG30700.023.598594
GCGAGTC30700.023.26016412
CGGGCGC8400.022.5858515
ATGTGTG32900.022.4412656
GACATGT33750.021.9768963
TGACATG33350.021.7232652
GGCGGGC9150.020.73521413
CGAGTCA35600.020.34867913
ACCCGTA34400.020.2671532
AAGGCGC35750.019.88178638
ACGGGTG35450.019.85013820
CCCGTAA36050.019.72217233
CGGGTGA35900.019.60131821
Produced by FastQC (version 0.11.2)