FastQCFastQC Report
Thu 7 Sep 2017
HVTL7BGX2_n01_tga1ox_20n_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTL7BGX2_n01_tga1ox_20n_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9343951
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG211860.22673492187619562No Hit
GTTTTACCCTACTGATGCCCGCGTCGCGATAGTAATTCAACCTAGTACGAGAGGAACCGTTGATTCGCACAATTG127230.13616295719016508No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGG125340.13414025822695347No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAG115130.12321340298124422No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTG111040.11883623961641065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG41300.039.0927548
TGTGTGC49700.032.9018367
CTGACAT50750.032.8450281
TGTGCGA50250.032.74759
GTGCGAG51900.031.50698310
ACATGTG54750.030.7500274
TGCGAGT52950.030.62175611
GCCAGTA30850.030.4279231
GACATGT58350.028.6754783
ATGTGTG59800.027.8087356
GCGAGTC59550.027.40096912
TGACATG60700.026.9409792
CGGGCGC14800.025.40511315
GGCGGGC15350.024.7204813
CGAGTCA67450.024.2420313
ACGGGTG71450.022.88390420
CGGGTGA73350.022.2441121
ACCCGTA73600.022.2277932
AACGGGT74200.022.0822719
CCAGTAG43800.021.4248872
Produced by FastQC (version 0.11.2)