FastQCFastQC Report
Thu 7 Sep 2017
HVTL7BGX2_n01_tga1ox_10n_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTL7BGX2_n01_tga1ox_10n_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18908956
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG713540.37735557690228905No Hit
GTTTTACCCTACTGATGCCCGCGTCGCGATAGTAATTCAACCTAGTACGAGAGGAACCGTTGATTCGCACAATTG420670.22247129878561248No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGG389960.20623031752784235No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAG363190.19207300498240093No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTG347420.1837330416338163No Hit
AAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTTA281230.14872846496654812No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTGCGAATGG256050.135412023805016No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGT237220.12545377967985116No Hit
GGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTTAGCC230370.12183115767999038No Hit
CGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGC229420.12132875024935275No Hit
GTTAGTTTTACCCTACTGATGCCCGCGTCGCGATAGTAATTCAACCTAGTACGAGAGGAACCGTTGATTCGCACA223270.11807632319838282No Hit
GTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGGTGCACCGCC221130.11694458435463069No Hit
CCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGTCG221070.11691285335901146No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTACGTGGAGGCATCCCTTCAC218600.11560659403935362No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGA204020.10789596210388347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACAT136350.041.6397361
GTGTGCG135400.041.021168
TGTGTGC143350.038.8423467
ACATGTG148450.037.952274
GCCAGTA71800.037.734831
GACATGT152400.037.080913
TGACATG153300.036.616432
ATGTGTG156250.035.971086
TGTGCGA156150.035.657879
GTGCGAG163650.033.89711410
TGCGAGT169100.032.86531411
GCGAGTC186450.029.88066112
CGAGTCA198450.028.1424613
CCAGTAG100200.026.684842
ACGGGTG228050.024.33770420
CAGTAGT112100.024.1901383
CCCTACT134450.024.093757
AACGGGT232350.023.85760719
CGGGCGC51350.023.84768115
GGCGGGC51700.023.82012213
Produced by FastQC (version 0.11.2)