FastQCFastQC Report
Thu 7 Sep 2017
HVTL7BGX2_n01_cotylset1_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTL7BGX2_n01_cotylset1_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13359766
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC382300.2861577066544429No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTA233840.17503300581761685No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC195690.1464771164405125No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATC191880.14362527008332332No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCT158880.11892423864310199No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAACCTCTGTAGGAC153630.11499452909579404No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTAC136580.10223232951834635No Hit
ATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAA134400.10060056441108325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG69550.028.3245335
CTAGTTG66900.026.5570958
TCTAGTT72100.025.9815857
CTCTAGT73950.025.1471636
TACTCTA73150.024.7135014
GGACACC68150.024.61154729
GTTACCT75300.023.179714
ACCTCTA75200.023.16465417
GACACCT75200.022.44256630
CTAATGC78250.022.26166721
TAGTTGT86650.021.4590199
TTTTTCC203600.021.3427621
GCTGGAC80300.021.26767326
TACCTCT82400.021.1824216
ATGCTGG81050.021.1101224
GTACTCT91250.020.3782423
TGTACTC90100.020.370922
TGTTACC86750.020.31944513
TTACCTC87200.020.17464815
TGGACAC84400.020.11731528
Produced by FastQC (version 0.11.2)