FastQCFastQC Report
Thu 7 Sep 2017
HVTL7BGX2_n01_cotylset1_16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTL7BGX2_n01_cotylset1_16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30186138
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC573360.1899414890371203No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTA329340.10910305915914119No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC309550.10254706978415058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTTG114300.022.6668038
ACTCTAG143000.022.4856785
TACTCTA124150.020.7307644
GGACACC116350.020.40740229
TCTAGTT140200.019.8819277
CTCTAGT143250.018.473046
ACCTCTA135450.018.43805517
GACACCT132250.018.21523730
GTTACCT142250.017.8720214
CACCTTA349550.017.8041977
ACCTTAG350450.017.6699358
CTAATGC140700.017.50478621
GTACTCT159200.016.4480083
CCTTAGA379350.016.4234439
GCTGGAC147700.016.2106926
TTTTTCC396750.015.9721431
TGGACAC151950.015.89785728
TAGTTGT172250.015.86189
TACCTCT158100.015.77473316
ATGCTGG154350.015.71104224
Produced by FastQC (version 0.11.2)