FastQCFastQC Report
Thu 7 Sep 2017
HVTL7BGX2_n01_cotylset1_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTL7BGX2_n01_cotylset1_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32572511
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC718200.22049267248693233No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTA412770.126723420248442No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATC381530.11713251090774057No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC380730.11688690503473927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG150400.024.5909395
CTAGTTG133650.022.8702148
TACTCTA142850.021.7362084
TCTAGTT155950.020.9935077
GGACACC139000.020.70704529
CTCTAGT159900.020.1314336
ACCTCTA155850.018.9460417
GTTACCT159600.018.86856314
CACCTTA434550.018.7201027
ACCTTAG439700.018.4930538
TTTTTCC447200.018.4226251
GACACCT156850.018.2629330
CTAATGC162950.018.16272721
TAGTTGT187000.017.230729
CCTTAGA481450.017.0395779
TTTCCAC489000.017.0310963
TTTTCCA512200.016.9605662
ATGCTGG174700.016.92178224
GTACTCT187600.016.7716643
GCTGGAC175850.016.5587526
Produced by FastQC (version 0.11.2)