FastQCFastQC Report
Thu 7 Sep 2017
HVTL7BGX2_n01_cotylset1_14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTL7BGX2_n01_cotylset1_14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37669969
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC757990.20121864183110955No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTA414320.10998681735044699No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC397710.10557746941602209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG170600.021.9426175
CTAGTTG141900.021.005438
CACCTTA431800.019.3983657
GGACACC143850.019.26528529
TACTCTA156250.019.2101424
TCTAGTT170850.018.9403027
ACCTTAG442600.018.9328658
CTCTAGT170700.018.656056
CCTTAGA479000.017.781749
TTTTTCC498800.017.2637521
GTTACCT174500.017.1584414
GACACCT162450.017.08096530
ACCTCTA171300.016.75394817
TTTCCAC527200.016.3339313
TTTTCCA562950.016.105952
CTAATGC179550.015.96488121
TCCACCT527400.015.9358385
TAGAGTA534500.015.85091612
TAGTTGT206650.015.591949
CCACCTT542600.015.5217096
Produced by FastQC (version 0.11.2)