FastQCFastQC Report
Thu 7 Sep 2017
HVTL7BGX2_n01_cotylset1_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTL7BGX2_n01_cotylset1_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24041426
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC469970.19548341267277575No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC259990.10814250369341652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG106100.023.6073635
CTAGTTG93250.021.7899678
GGACACC90200.021.12001829
CACCTTA254800.020.0514497
CTCTAGT104000.020.0041126
ACCTTAG261500.019.4849328
TCTAGTT109450.019.4788287
TACTCTA107850.019.3852924
GTTACCT108150.018.69079614
ACCTCTA109750.018.44958517
GACACCT105700.018.28468330
CCTTAGA287150.017.9362669
CTAATGC113900.017.35341321
TAGTTGT130300.016.33519
TACCTCT123000.016.29393216
TTTCCAC321150.016.2319953
TTTTTCC316000.016.2295041
GCTGGAC119650.016.17596226
TAGAGTA317400.016.16125912
TTACCTC127200.015.94582215
Produced by FastQC (version 0.11.2)