FastQCFastQC Report
Thu 7 Sep 2017
HVTL7BGX2_n01_cotylset1_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTL7BGX2_n01_cotylset1_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14240042
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC508110.3568177678127635No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTA285500.20049098169794724No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC250090.1756244820064435No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATC189090.13278752969970173No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCT176670.12406564531200119No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTAC150730.10584940690483918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG83450.032.1242835
CTAGTTG77000.031.7200628
TCTAGTT84350.030.3868797
CTCTAGT85950.029.3844596
ACCTCTA82850.028.72739417
GGACACC81700.028.47273329
GTTACCT87200.027.73022314
TACTCTA90850.027.2289854
CTAATGC89600.026.6401221
GACACCT89950.026.16851630
TACCTCT93350.025.64412116
TTTTTCC227750.025.4614681
TAGTTGT99100.025.1326489
GCTGGAC94850.024.87918726
CCTCTAA97700.024.5373918
ATGCTGG97600.024.42167724
TGTTACC100900.024.23873513
TTACCTC101300.024.07424415
TGGACAC98100.023.9571928
TAATGCT104650.022.90789622
Produced by FastQC (version 0.11.2)