FastQCFastQC Report
Thu 7 Sep 2017
HVTL7BGX2_n01_cotylset1_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTL7BGX2_n01_cotylset1_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13785175
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC375820.27262620895273365No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTA223760.1623193031644502No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC191590.13898263895815613No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATC160950.11675586272934511No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCT147400.10692646266732195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG75150.030.2097825
CTAGTTG65950.028.4054078
TCTAGTT72450.027.3807587
GGACACC67250.026.9939929
ACCTCTA73100.025.43447717
CTCTAGT75450.025.3802226
GACACCT73800.024.69197830
TACTCTA77850.024.6411274
GTTACCT77000.024.32551814
CTAATGC79400.023.37284921
ATGCTGG80200.022.92481424
TACCTCT84150.022.01261316
GCTGGAC84050.021.79666926
TTTTTCC200200.021.7928161
TGTTACC88450.021.48907913
CCTCTAA88150.021.36584918
TAGTTGT90950.021.2789889
TGGACAC86050.021.21572728
TTACCTC90000.020.96506515
CACCTTA208650.020.5526947
Produced by FastQC (version 0.11.2)