FastQCFastQC Report
Thu 7 Sep 2017
HVTL7BGX2_n01_cotylset1_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTL7BGX2_n01_cotylset1_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35725789
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC523020.14639844623165635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG147800.019.4206775
CACCTTA322050.018.681227
ACCTTAG328500.018.3144458
CTAGTTG122850.018.2524388
GGACACC114350.017.83757629
CCTTAGA355150.017.056579
TCTAGTT152850.015.9564427
ACCTCTA139450.015.7073617
TACTCTA142950.015.5903754
TAGAGTA392700.015.5131412
GACACCT133450.015.46606730
CTCTAGT155250.015.0445946
GTTACCT147250.014.85212714
TTAGAGT420800.014.40367211
CTAATGC147750.014.38128121
TCCACCT421150.014.3274365
CTTAGAG423100.014.2684410
CCACCTT426250.014.140086
TTTCCAC437800.014.0973453
TTTTTCC444150.013.777651
Produced by FastQC (version 0.11.2)