FastQCFastQC Report
Thu 7 Sep 2017
HVTL7BGX2_n01_cotylset1_09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTL7BGX2_n01_cotylset1_09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16813440
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC1152230.6853029481176963No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTA657190.3908718263484451No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC578620.3441413535837996No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATC445180.26477627421872024No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCT378460.22509373453618056No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCT336280.2000066613375966No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTAC328150.1951712439572152No Hit
ATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAA314220.1868862053214571No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCA292030.17368843020821437No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAACCTCTGTAGGAC268230.1595330878154619No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATC265690.1580223916105211No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTA245080.14576434090822582No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC228200.13572475353050895No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTA176540.10499933386624034No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCT172690.10270949906741275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG133250.040.027685
TCTAGTT132900.037.404367
CTAGTTG130250.037.2123458
CTCTAGT133450.037.124936
TTTTTCC360550.034.902411
GTTACCT145550.033.2036614
ACCTCTA144900.033.11375417
TACTCTA150000.032.682834
GGACACC144850.032.09331529
TAGTTGT154100.031.9898979
CTAATGC152550.031.27200921
TGTTACC156550.031.06937613
TTACCTC156350.030.99797415
TACCTCT155950.030.76762816
TTTTCCA414950.030.3889872
GACACCT156350.030.08637430
ATGCTGG157750.029.84793524
CCTCTAA161100.029.82670218
GTACTCT168000.029.344953
GCTGGAC162000.029.02661526
Produced by FastQC (version 0.11.2)