FastQCFastQC Report
Thu 7 Sep 2017
HVTL7BGX2_n01_cotylset1_08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTL7BGX2_n01_cotylset1_08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25725564
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC675490.26257538998950614No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTA451820.17563074613252405No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC370000.14382580688998692No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATC285580.11101019981524993No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCT269500.10475960799149048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG144200.031.151115
CTAGTTG124950.030.6743978
GGACACC127000.028.20807329
TCTAGTT142100.028.0402537
CTCTAGT142750.027.7946056
ACCTCTA137650.027.03913917
CTAATGC141150.026.39289921
GTTACCT143450.026.28299314
GACACCT141250.025.58280630
TACTCTA154500.025.1214734
TAGTTGT163550.024.0247929
TACCTCT159500.023.5081816
ATGCTGG155550.023.46222324
GCTGGAC156500.023.3458826
TGGACAC160000.022.81988128
CCTCTAA165150.022.74548518
TTACCTC167500.022.67375815
TGTTACC168050.022.6001213
CTGGACA167200.021.89691427
TTTTTCC370200.021.8337211
Produced by FastQC (version 0.11.2)