FastQCFastQC Report
Thu 7 Sep 2017
HVTL7BGX2_n01_cotylset1_07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTL7BGX2_n01_cotylset1_07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30378946
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC648100.21333854044837502No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTA467840.1540013929383857No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC346370.11401646390233552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG153750.028.2505345
CTAGTTG130400.027.116828
GGACACC134750.025.150829
CTCTAGT148000.024.8965916
TCTAGTT154350.024.4512887
GACACCT142200.023.71224430
GTTACCT152450.023.30597114
ACCTCTA149950.023.28022417
CTAATGC152900.022.65054721
TACTCTA161250.022.4007024
TACCTCT167650.020.9664316
TAGTTGT175450.020.8025659
ATGCTGG170900.020.36613724
TTACCTC178150.020.29227615
GCTGGAC172200.020.055226
TGTTACC181050.019.81524313
TGGACAC174300.019.68029428
CCTCTAA180150.019.54987118
TAATGCT189550.018.47118222
CTGGACA187550.018.32470527
Produced by FastQC (version 0.11.2)