FastQCFastQC Report
Thu 7 Sep 2017
HVTL7BGX2_n01_cotylset1_06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTL7BGX2_n01_cotylset1_06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21049760
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC496680.2359551842871368No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC274030.13018200682573103No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTA270600.12855253456571478No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATC244450.11612959007608638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG105650.028.4095445
CTAGTTG98050.027.373618
GGACACC97900.025.69875329
CTCTAGT108800.025.2732126
TCTAGTT112050.024.9077597
TACTCTA112900.024.4765384
ACCTCTA108600.023.9811917
GACACCT106950.023.62135130
CTAATGC112200.023.08876621
GTTACCT114600.022.84624914
GCTGGAC115250.022.27187726
TAGTTGT126400.021.861529
ATGCTGG118600.021.40665424
TACCTCT122200.021.34053216
TGGACAC120900.021.03669228
CACCTTA272650.020.611697
ACCTTAG274600.020.440298
TTACCTC128150.020.43049415
TGTTACC130850.020.40479513
CCTCTAA130950.019.88818218
Produced by FastQC (version 0.11.2)