FastQCFastQC Report
Thu 7 Sep 2017
HVTL7BGX2_n01_cotylset1_05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTL7BGX2_n01_cotylset1_05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28814631
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC622000.2158625595448368No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTA360530.12512046397540194No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATC325570.11298773876368572No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC324310.11255046090994536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG133600.024.0942555
CTAGTTG114750.021.8262588
CTCTAGT129250.020.5277296
TCTAGTT133300.020.0827487
TACTCTA127450.019.9780254
GGACACC118300.019.66290529
CACCTTA364050.019.0564777
ACCTTAG372200.018.6671688
ACCTCTA134550.018.04844717
GACACCT130200.018.02554930
GTTACCT138000.017.89746914
TTTTTCC394800.017.8249861
CCTTAGA399450.017.5487469
CTAATGC138450.017.46520621
TTTTCCA439500.017.1131172
TTTCCAC423050.016.9057563
TACCTCT154350.015.95670616
TAGAGTA440250.015.89055512
GTACTCT162450.015.8433183
ATGCTGG152200.015.81954224
Produced by FastQC (version 0.11.2)