FastQCFastQC Report
Thu 7 Sep 2017
HVTL7BGX2_n01_cotylset1_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTL7BGX2_n01_cotylset1_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24340102
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC507780.20861868204167755No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTA261730.10753036285550488No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATC260220.10690998747663424No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC256840.10552133265505625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG108500.021.9728725
CACCTTA273500.020.4465687
ACCTTAG277100.020.0938268
CTAGTTG97200.019.662528
TACTCTA102100.018.7881094
CCTTAGA302050.018.5934729
TCTAGTT110950.018.4383537
CTCTAGT110000.018.0669276
TTTTTCC317400.017.9106271
GGACACC98950.017.75339729
TTTCCAC338450.016.9317553
ACCTCTA110000.016.90238417
TAGAGTA335950.016.78877812
TCCACCT340100.016.5558685
CCACCTT339650.016.5073096
GTTACCT113350.016.49420714
GACACCT108000.016.42581430
TTTTCCA364350.016.3441492
CTAATGC115900.015.80375721
CTTAGAG357800.015.74456910
Produced by FastQC (version 0.11.2)