FastQCFastQC Report
Thu 7 Sep 2017
HVTL7BGX2_n01_cotylset1_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTL7BGX2_n01_cotylset1_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46554468
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC1061140.2279351575878818No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTA608070.13061474572107665No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATC600240.1289328448560512No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC541360.11628529403450599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG227700.026.0004065
CTAGTTG201800.024.6341938
GGACACC203100.022.85452329
TCTAGTT232850.022.6973557
CTCTAGT229200.022.3988556
TACTCTA230700.022.0131344
ACCTCTA226400.021.51367817
CTAATGC231450.020.64184421
GTTACCT238150.020.55398814
GACACCT230250.020.130130
TTTTTCC634650.019.0340771
ATGCTGG252450.018.84301624
CACCTTA631700.018.6224257
TACCTCT261650.018.58906616
GCTGGAC252750.018.57737726
ACCTTAG643550.018.2742798
TAGTTGT282050.018.248779
CCTCTAA269700.018.08524318
TGGACAC261350.018.01010328
GTACTCT282400.017.9340113
Produced by FastQC (version 0.11.2)