FastQCFastQC Report
Thu 7 Sep 2017
HVTL7BGX2_n01_cotylset1_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTL7BGX2_n01_cotylset1_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25916298
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC598920.23109782114714067No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC427110.16480363051852545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCTTA202100.023.0607537
ACTCTAG123700.022.6472785
GGACACC95800.022.407629
ACCTTAG209350.022.179838
CTAGTTG103150.021.9726418
GACACCT109400.019.93782430
CTCTAGT120950.019.197436
GTGTAGG289250.019.16454315
GTTACCT115600.019.1275214
ACCTCTA115700.018.99154917
CCTTAGA250450.018.8013639
CTAATGC118950.018.7335421
TCTAGTT123650.018.72037
AGGGTAG302300.018.28003319
GGGTAGT307350.018.08065220
AGTGTAG316950.017.69653514
GTATGAG316650.017.6487229
TACTCTA128900.017.5581254
GAGTGTA318100.017.5461113
TCCACCT267300.017.5408335
Produced by FastQC (version 0.11.2)