FastQCFastQC Report
Thu 7 Sep 2017
HVTL7BGX2_n01_cotylset1_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTL7BGX2_n01_cotylset1_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20494217
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC325810.15897655421526963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG83050.021.352315
CTAGTTG70700.019.3225468
CTCTAGT82050.018.5437856
GGACACC71200.018.03215229
TCTAGTT86700.017.825657
CACCTTA202850.017.7376377
ACCTTAG210050.017.1297178
TACTCTA82150.016.6303184
CCTTAGA228900.015.8544479
GACACCT81200.015.64169730
ACCTCTA86250.015.55734917
GTTACCT89000.015.15438214
CTAATGC87850.014.76345421
TTTCCAC255700.014.6523313
TTTTTCC252350.014.5524351
TAGAGTA251100.014.53485112
TTTTCCA281550.014.0304082
TAGTTGT104950.014.0025889
TGGACAC94750.013.94984128
TTAGAGT265900.013.75202211
Produced by FastQC (version 0.11.2)