FastQCFastQC Report
Thu 7 Sep 2017
HVTL7BGX2_n01_col_60n_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTL7BGX2_n01_col_60n_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5931548
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG211300.3562307849485497No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTACGTGGAGGCATCCCTTCAC174620.2943919529943954No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTG173240.2920654102436666No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGG158860.2678221604208547No Hit
GCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGG150340.2534582877859203No Hit
GTTTTACCCTACTGATGCCCGCGTCGCGATAGTAATTCAACCTAGTACGAGAGGAACCGTTGATTCGCACAATTG145580.2454334011964499No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAG116210.1959185022189823No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTGCGAATGG111430.18785989761863175No Hit
CGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGC107890.18189180969284915No Hit
CCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGTCG98030.16526883032894618No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGT90430.15245598619449763No Hit
GTTAGTTTTACCCTACTGATGCCCGCGTCGCGATAGTAATTCAACCTAGTACGAGAGGAACCGTTGATTCGCACA87610.14770174666039962No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGA81800.1379066644997225No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT79740.13443370937906934No Hit
TATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTTAGCCAACGACACGTGCCTTTGGGAGCCTAAG79680.13433255534642896No Hit
AAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTTA78330.13205658961202033No Hit
GAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGTGTGAACTCGTTGAATACATCAGTGTAGCGCGCGTGCGG78040.13156767845425849No Hit
GGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTTAGCC72250.1218063143044615No Hit
GTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGGTGCACCGCC71140.11993496470061442No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTGCGAATGGCTCATTAA69630.11738925487916477No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAGC68660.11575393135147857No Hit
TTATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTTAGCCAACGACACGTGCCTTTGGGAGCCTAA65340.11015674154537737No Hit
GTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTGCGAA64360.10850455901225109No Hit
CTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCCGTATT59710.1006651214826214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA31800.041.2397421
CTGACAT37600.040.844321
GTGTGCG39950.037.564868
TGTGTGC41950.036.2670567
TGACATG42350.036.1707232
ACATGTG44650.034.694444
GACATGT45400.034.196423
CCAGTAG40000.032.603142
ATGTGTG47250.032.5670936
TGTGCGA47200.031.7209429
GTGCGAG49150.030.46217510
TGCGAGT51500.029.13864711
CAGTAGT46600.028.20743
CGGGATT48450.026.845732
GGGATTA48600.026.2657263
AGTAGTC51400.025.7752634
GCGAGTC59000.025.49262212
ACCCGCT51400.024.6992119
GCGATTT58300.024.3294751
CGAGTCA61800.024.2813913
Produced by FastQC (version 0.11.2)