FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_04_09_gr_dam_bzip1_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_04_09_gr_dam_bzip1_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19187393
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG504940.2631623795895565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGATC2210550.064.265111
AGGATCT754150.064.20425412
GGTCGCG2229000.063.8394051
AGGATCA674500.063.74345412
CCGAGGA2260550.063.114759
GGATCTT219900.062.65936313
CGGCCGA2298700.062.6467136
CGAGGAT2274600.062.62642310
GGCCGAG2301450.062.5937927
GTCGCGG2294850.062.128462
GGATCTA74350.062.08827213
GGATCAA172900.061.87941713
GGATCTC244000.061.79191613
GGATCAT201950.061.50464613
GCCGAGG2342950.061.4014368
AGGATCG283050.061.34066412
CGCGGCC2362300.060.612194
GGATCCA173050.060.57187313
GGATCTG248750.060.5697613
TCGCGGC2358300.060.4908753
Produced by FastQC (version 0.11.2)