FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_04_09_gr_dam_bzip1_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_04_09_gr_dam_bzip1_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19013897
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG501500.2637544528615044No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG2293700.062.5586741
GAGGATC2289350.062.54658511
AGGATCT780300.062.4247512
AGGATCA711050.062.00241512
CCGAGGA2345100.061.4277659
CGGCCGA2363700.061.403046
GGCCGAG2370900.061.254477
CGAGGAT2346200.061.2385510
GTCGCGG2352250.061.0911642
GGATCTT234050.060.8613
GCCGAGG2414500.060.122128
GGATCAA181000.060.11575313
GGATCAT226100.059.9195813
CGCGGCC2422200.059.6035734
TCGCGGC2416150.059.5253643
GGATCTA83000.059.45483413
GGATCTC245450.059.38792413
CTAATAC135300.059.3860051
GGATCCG106050.059.3039413
GGATCCA191550.058.99728813
Produced by FastQC (version 0.11.2)