FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_04_09_gr_dam_bzip1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_04_09_gr_dam_bzip1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21852653
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGATC2581200.065.4746611
AGGATCT868600.065.18143512
AGGATCA774400.064.62736512
GGTCGCG2608150.064.515361
CCGAGGA2631650.064.367699
CGAGGAT2649900.063.93832810
CGGCCGA2675300.063.8076556
GGCCGAG2678700.063.7411467
GGATCTT244050.063.4003113
GTCGCGG2689200.062.7636832
AGGATCG340850.062.6651112
GGATCAT238350.062.64052613
GGATCCA221800.062.6123213
GCCGAGG2728200.062.58928
GGATCAA187950.062.47408713
GGATCTA84650.062.3482213
GGATCTC298250.062.07617613
GGATCCC142350.061.49020413
GGATCTG284650.061.46411513
CGCGGCC2761400.061.4595534
Produced by FastQC (version 0.11.2)