FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_04_09_gr_dam_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_04_09_gr_dam_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21559219
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATCT977450.066.5124312
GAGGATC2907500.066.509611
AGGATCA862350.066.35181412
GGATCTT286750.065.51810513
CCGAGGA2967400.065.385389
AGGATCG351150.065.29201512
CGAGGAT2977800.065.1182410
GGATCTC313650.065.087713
GGTCGCG2973600.064.999421
GGATCAA207200.064.87939513
GGATCCA247800.064.6162913
CGGCCGA3038450.064.607726
GGCCGAG3043550.064.545787
GGATCGT94850.064.4994913
GGATCAT266000.064.4976513
GGATCTA95850.064.4473113
GGATCCC156500.063.9593313
GCCGAGG3081700.063.698738
GGATCTG310400.063.6381413
GTCGCGG3057150.063.4385532
Produced by FastQC (version 0.11.2)