FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_04_09_gr_dam_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_04_09_gr_dam_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32725184
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATCT1465450.066.9441612
GAGGATC4368300.066.6733911
AGGATCA1272150.066.6564612
GGATCTA132500.065.8497513
AGGATCG531000.065.8373912
GGATCTT419500.065.8002613
GGATCCA377000.065.61497513
GGATCTC486950.065.58375513
CCGAGGA4461150.065.561099
CGAGGAT4468550.065.3617610
GGATCAA290950.065.3054213
GGATCCC239450.065.0564713
GGTCGCG4523150.064.8939061
GGATCAT383800.064.8445513
GGATCTG464200.064.6587713
CGGCCGA4632700.064.462996
GGCCGAG4637450.064.39447
GGATCCG192800.063.6977513
GCCGAGG4686000.063.6973658
GGATCAC303100.063.4381413
Produced by FastQC (version 0.11.2)