FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_04_09_gr_dam_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_04_09_gr_dam_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29094822
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATCT1195400.067.0509312
GAGGATC3655950.066.8901611
AGGATCA1060400.066.7782812
AGGATCG478050.066.24055512
GGATCTC395750.065.74364513
GGATCAA240150.065.7127613
CCGAGGA3738450.065.615539
GGATCTT329700.065.60268413
GGATCTA109650.065.4969113
GGATCCA313100.065.4032213
CGAGGAT3748650.065.3891610
GGTCGCG3774450.065.02091
GGATCCC195850.064.7972213
CGGCCGA3854250.064.743046
GGCCGAG3860300.064.6896747
GGATCTG396200.064.4234313
GGATCAT317750.064.3910313
GGATCCG172400.064.19137613
GGATCGT128850.064.13035613
GGATCAC251500.063.74915313
Produced by FastQC (version 0.11.2)