FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_03_26_gr_dam_bzip1_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_03_26_gr_dam_bzip1_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13908232
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATCT578800.066.3805412
GAGGATC1903800.066.1185211
GGTCGCG1964650.065.805461
AGGATCA575200.065.7555512
CCGAGGA1928550.065.493919
GGATCTA46200.065.4514713
AGGATCG282650.065.2045612
CGAGGAT1934650.065.1944410
CGGCCGA2044750.064.402746
GGATCTT159950.064.3514613
GGATCTC190450.064.2815713
GGCCGAG2056800.064.0310757
GCCGAGG2064450.063.7361568
GGATCCA154050.063.68087813
GGATCAA125150.063.6486313
GTCGCGG2047100.063.4099852
GGATCAT160150.062.7850713
GGATCCC103050.062.59288813
GGATCTG206900.062.52002313
GGATCCG100250.062.1068113
Produced by FastQC (version 0.11.2)