FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_03_26_gr_dam_bzip1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_03_26_gr_dam_bzip1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12979058
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG1651250.065.83031
GAGGATC1611000.065.5510211
AGGATCT498200.065.48559612
CCGAGGA1627150.065.105249
AGGATCA484150.064.8993112
AGGATCG239250.064.7733112
CGAGGAT1635650.064.69176510
CGGCCGA1732350.063.921156
GGATCTA44150.063.49563613
GGCCGAG1745600.063.468777
GGATCTT139300.063.212313
GTCGCGG1731300.063.068542
GCCGAGG1754050.063.0590178
GGATCAA110400.062.98989513
GGATCCA134800.062.80410813
GGATCTC163100.062.7215713
GGATCCC84200.061.89060213
TCGCGGC1778400.061.5040173
GGATCCG87800.061.42571313
CGCGGCC1795350.061.3798144
Produced by FastQC (version 0.11.2)