FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_03_26_gr_dam_bzip1_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_03_26_gr_dam_bzip1_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24351382
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATCT1009600.066.9146612
GAGGATC3226750.066.7928311
AGGATCA1014450.066.6809912
CGAGGAT3289250.065.6600110
CCGAGGA3297300.065.6038749
GGATCTA93700.065.5149313
GGATCAA249600.065.4540513
AGGATCG421400.065.43684412
GGTCGCG3338850.065.417821
GGATCTC316000.065.36758413
GGATCTT285700.065.3053413
GGATCCA269500.065.15317513
CGGCCGA3439850.064.6314856
GGATCAT297050.064.56560513
GGCCGAG3450900.064.453727
GGATCCC166650.064.431813
GGATCTG346350.064.3181413
GCCGAGG3475600.063.9563988
GTCGCGG3439550.063.7079962
GGATCAC239650.063.48373813
Produced by FastQC (version 0.11.2)