FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_03_26_gr_dam_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_03_26_gr_dam_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15290981
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA221410.14479777327563223No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGATC1849950.066.88428511
AGGATCT590200.066.8770412
AGGATCA575900.066.7205912
AGGATCG243950.066.3808512
CGAGGAT1877050.066.0141710
GGATCAA155750.065.50265513
GGTCGCG1922200.065.462511
CCGAGGA1900300.065.316999
GGATCTC174000.065.16946413
CGGCCGA1976850.064.862626
GGATCCA158300.064.8454613
GGATCTT186150.064.751313
GGCCGAG1988800.064.5085147
GCCGAGG2000450.064.087148
GGATCAT170550.064.08681513
GGATCTA66500.063.89184613
GTCGCGG1979500.063.8372152
GGATCTG186750.063.77489513
GGATCCC89050.063.5906113
GGATCCG91800.063.2106513
Produced by FastQC (version 0.11.2)