FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_03_26_gr_dam_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_03_26_gr_dam_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16520934
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATCT585850.066.42319512
GAGGATC1845650.065.76546511
AGGATCA528750.065.7329412
GGTCGCG1897850.065.5933151
AGGATCG276900.065.003112
CCGAGGA1872550.064.948599
CGAGGAT1873450.064.8717710
GGATCTA57100.064.7256113
GGATCTT184550.064.3075713
CGGCCGA1974700.064.155486
GGATCTC189050.063.81355713
GGCCGAG1992550.063.6261337
GGATCAA126050.063.52746613
GCCGAGG1995500.063.4428258
GGATCCA149500.063.0439713
GTCGCGG1985350.062.9378662
CGCGGCC2026800.062.1607444
GGATCCC97300.062.1194913
GGATCAC124700.062.08218813
GGATCTG183550.061.98847613
Produced by FastQC (version 0.11.2)