FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_03_26_gr_dam_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_03_26_gr_dam_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26360061
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATCT989650.066.5759412
GAGGATC3193950.066.2520711
AGGATCA980650.065.9414212
GGTCGCG3307100.065.446461
GGATCTA90050.065.2546113
CCGAGGA3259400.065.186919
CGAGGAT3254250.065.18001610
AGGATCG443100.065.169712
GGATCTC316200.064.9711313
GGATCAA238200.064.5451613
CGGCCGA3427750.064.381326
GGATCTT278850.064.3733713
GGCCGAG3446600.064.024977
GCCGAGG3472850.063.492668
GGATCCA268600.063.41610313
GTCGCGG3430850.063.367272
GGATCCC170250.063.0890513
GGATCTG345900.062.58979813
GGATCAT282300.062.3097213
GGATCAC240750.062.27695513
Produced by FastQC (version 0.11.2)