FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_03_12_gr_only_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_03_12_gr_only_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25882324
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATCT1085250.067.25136612
AGGATCA1000900.067.1809312
GAGGATC3409700.067.1211111
GGATCAA217800.066.5572613
GGATCTC355600.066.3446313
AGGATCG478450.066.3017312
GGATCTA99500.066.1268713
CCGAGGA3474450.066.0909659
GGATCTT278400.066.0109513
GGATCCA280200.065.9990813
GGATCAT276050.065.73614513
CGAGGAT3494350.065.6703910
GGATCTG374550.065.538913
GGATCCC181850.065.3770713
GGATCAG292100.065.35914613
GGTCGCG3509300.065.26471
GGATCCG169350.065.2014713
CGGCCGA3587350.065.014936
GGCCGAG3591650.064.957397
GGATCAC240450.064.8871213
Produced by FastQC (version 0.11.2)