FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_03_12_gr_only_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_03_12_gr_only_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26366392
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA372630.14132764164319486No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATCT1016950.066.6986212
AGGATCA915100.066.59173612
AGGATCG420500.066.4499712
GAGGATC3152100.066.4472611
GGATCTC331250.065.96055613
GGATCCA255150.065.85412613
GGATCAA209750.065.69166613
GGATCTT271000.065.4894313
GGATCTG338750.065.3163713
GGATCTA95600.065.23740413
GGATCCC167650.065.02814513
CGAGGAT3231400.064.9923210
GGATCGT111600.064.9161913
GGATCAC211300.064.72945413
CCGAGGA3247800.064.707239
GGATCCG160900.064.6674513
GGATCAT270200.064.569413
GGATCAG250700.064.342713
GGTCGCG3277850.064.119041
CGGCCGA3349950.063.8230866
Produced by FastQC (version 0.11.2)