FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_03_12_gr_dam_bzip1_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_03_12_gr_dam_bzip1_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24888799
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATCT897300.067.3245612
GAGGATC2621550.067.2093511
AGGATCA806550.066.6941612
GGTCGCG2647500.066.539041
AGGATCG304250.066.3613412
GGATCTT329050.065.95592513
GGATCCA231400.065.8535313
GGATCTA119400.065.8353913
CGGCCGA2726750.065.829666
GGATCTC253450.065.8274213
CGAGGAT2689550.065.6233110
CCGAGGA2698250.065.4611369
GGCCGAG2745600.065.426187
GTCGCGG2706900.065.252592
GGATCAA250850.065.0447313
GCCGAGG2760750.065.044458
GGATCGT76750.064.571213
CGCGGCC2761200.064.558194
GGATCTG221350.064.5269413
GGATCCC112100.064.4091613