FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_03_12_gr_dam_bzip1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_03_12_gr_dam_bzip1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21407448
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGATC2473450.066.88618511
AGGATCT831450.066.88106512
AGGATCA742650.066.730112
AGGATCG308000.066.3370412
GGTCGCG2498300.065.878831
CCGAGGA2524500.065.7016759
GGATCTT270100.065.64284513
CGAGGAT2527250.065.5714110
GGATCAA202900.065.4600813
CGGCCGA2562750.065.3295756
GGATCTA95700.065.1692313
GGATCTC251300.065.1500213
GGATCCA215600.065.1429213
GGCCGAG2575250.065.071937
GGATCCC115400.064.6892113
GGATCCG115050.064.55138413
GGATCAT236300.064.5461513
GTCGCGG2561700.064.463032
GGATCTG238850.064.399213
GCCGAGG2602550.064.381128
Produced by FastQC (version 0.11.2)