FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_03_12_gr_dam_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_03_12_gr_dam_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20670554
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGATC2309800.066.7211111
AGGATCA690100.066.65439612
GGTCGCG2291600.066.6425861
AGGATCT806450.066.594112
AGGATCG268600.065.86603512
GGATCTT316600.065.796313
GGATCCA205100.065.67979413
CGGCCGA2375350.065.603486
CCGAGGA2354450.065.5809869
CGAGGAT2352900.065.56570410
GGATCAT219950.065.2711313
GTCGCGG2350100.065.226672
GGCCGAG2395250.065.1074457
GGATCTA104300.065.0643413
GGATCTG187800.065.0584613
GGATCTC214650.065.0566213
GGATCAC128800.064.9972213
GGATCAA214850.064.9146113
GGATCCG109600.064.7919413
CGCGGCC2391200.064.688644
Produced by FastQC (version 0.11.2)