FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_03_12_gr_dam_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_03_12_gr_dam_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17552026
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG1894550.066.900681
AGGATCT694100.066.6832412
GAGGATC1922200.066.6555211
AGGATCA580000.066.3513612
AGGATCG212250.066.0056312
CGGCCGA1973900.065.75626
CCGAGGA1957550.065.62929
GGATCTT277850.065.5629313
CGAGGAT1959450.065.4776710
GTCGCGG1941600.065.419782
GGCCGAG1986250.065.409197
GGATCTC189250.065.37347413
GCCGAGG1998400.065.0183268
CGCGGCC1985800.064.926424
GGATCTA97850.064.703213
GGATCCG83750.064.6059813
GGATCAA188250.064.512313
TCGCGGC1970650.064.496623
GCGGCCG2015150.064.443375
GGATCTG148050.064.0868813
Produced by FastQC (version 0.11.2)